The 2016 October 30th Central Italy earthquake (Mw 6,5) was the largest event occurred in Italy after the 1980 Irpinia event. Despite the great distance from the epicenter (> 100 km), damages to buildings were recorded even in the southern area of Rome. In particular the San Paolo basilica was temporarily closed for damage assessment. ISPRA experts were immediately claimed to implement a damage assessment in the Necropolis of the via Ostiensis, located close to the church. The whole archaeological site was affected by a set of N-S trending parallel cracks induced by the earthquake. The relationship between the Necropolis and the neighboring Tiber's valley has been also investigated. Active and passive seismic surveys, as well as GPR surveys, helped in clarifying the local stratigraphic setting and its influence on local effects.